Optical Imaging  

Photo-acoustic Tomography


In photo-acoustic tomography, an expanded pulsed laser beam diffuses into biological tissue and generates a small but rapid temperature rise, which causes the emission of ultrasonic waves as a result of thermoelastic expansion. The short-wavelength ultrasonic waves are then detected to form high-resolution tomographic images.

Selected publications:

  • L. V. Wang and J. Yao, “A practical guide to photoacoustic tomography in the life sciences,” Nature Methods 13(8), 627-637(Aug. 2016) [PDF]

  • J. Yao, A. A. Kaberniuk, L. Li, D. M. Shcherbakova, R. Zhang, L. Wang, G. Li, V. V. Verkhusha, and L. V. Wang, “Multiscale photoacoustic tomography using reversibly switchable bacterial phytochrome as a near-infrared photochromic probe,” Nature Methods, (2016) doi:10.1038/nmeth.3656. [Request PDF]

  • J. Yao, L. Wang, J-M Yang, K. Maslov, T. Wong, L. Li, C-H Huang, J. Zou, and L. V. Wang, "High-speed Label-free Functional Photoacoustic Microscopy of Mouse Brain in Action", Nature Methods. Published in advanced online publication March 30, 2015, doi: 10.1038/nmeth.3336. [PDF]

  • P. Lai, L. Wang, J. Tay, and L. V. Wang, "Photoacoustically guided wavefront shaping (PAWS) for enhanced optical focusing in scattering media," Nature Photonics 9, advance online publication, doi:10.1038/nphoton.2014.322 (2015). [PDF]

  • L. Wang, C. Zhang, and L. V. Wang, “Grueneisen relaxation photoacoustic microscopy,” Physical Review Letters 113, 174301 (Oct. 2014).[PDF]

  • L. V. Wang, "Photoacoustic tomography," Scholarpedia, 9(2):10278(2014). [HTML]

  • J. Yao, L. Wang, C. Li, C. Zhang, and L. V. Wang, “Photoimprint Photoacoustic Microscopy for Three-Dimensional Label-Free Subdiffraction Imaging,” Physical Review Letters 112(1), 014302(Jan. 2014). [PDF]

  • M. Nasiriavanaki, J. Xia, H. Wan, A. Q. Bauer, J. P. Culver, and L. V. Wang, “High-resolution photoacoustic tomography of resting-state functional connectivity in the mouse brain,” Proceedings of the National Academy of Sciences 111(1), 21-26 (Jan.2014). [PDF]

  • L. Wang, J. Xia, J. Yao, K. I. Maslov, and L. V. Wang,"Ultrasonically Encoded Photoacoustic Flowgraphy in Biological Tissue," Physical Review Letters 111(20), 204301(Nov. 2013)[PDF]

  • L. Wang, K. Maslov, and L. V. Wang, "Single-cell label-free photoacoustic flowoxigraphy in vivo," Proceedings of the National Academy of Sciences 110(15), 5759-5764 (Mar. 2013)[PDF]

  • J.-M. Yang, C. Favazza, R. Chen, J. Yao, X. Cai, K. Maslov, Q. Zhou, K. K. Shung, and L. V. Wang, “Simultaneous functional photoacoustic and ultrasonic endoscopy of internal organs in vivo,” Nature Medicine 18(8), 1297-1302 (Aug. 2012). [PDF]

  • L. V. Wang and S. Hu, “Photoacoustic tomography: in vivo imaging from organelles to organs,” Science 335, 1458–1462 (Mar. 2012). [PDF]

  • L. V. Wang, “Multiscale photoacoustic microscopy and computed tomography,” Nature Photonics 3 (9), 503–509 (Sep. 2009). [PDF]

  • [Z. Guo, L. Li], and L. V. Wang, “On the speckle-free nature of photoacoustic tomography,” Medical Physics 36 (9), 4084–4088 (Aug. 2009). [PDF]

  • L. V. Wang, "Prospects of photoacoustic tomography," Medical Physics 35 (12), 5758–5767 (Dec. 2008).[PDF]

  • K. H. Song, E. W. Stein, J. A. Margenthaler, and L. V. Wang, "Noninvasive photoacoustic identification of sentinel lymph nodes containing methylene blue in vivo in a rat model," Journal of Biomedical Optics 13, 054033-(1–6) (Sep. 2008).[PDF]

  • K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, "Optical-resolution photoacoustic microscopy for in vivo imaging of single capillaries," Optics Letters 33, 929–931 (Apr. 2008). [PDF]

  • H. F. Zhang, K. Maslov, and L. V. Wang, "In vivo imaging of subcutaneous structures using functional photoacoustic microscopy," Nature Protocols 2(4), 797–804 (Apr. 2007).[PDF]

  • H. F. Zhang, K. Maslov, G. Stoica, and L.-H. Wang, "Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging," Nature Biotechnology 24, 848–851 (Jul. 2006).[PDF]

  • M. Xu and L.-H. Wang, "Biomedical photoacoustics," Review of Scientific Instruments 77 (4), 041101 (Apr. 2006).[PDF]

  • M. Xu and L.-H. Wang, "Universal back-projection algorithm for photoacoustic-computed tomography," Physical Review E 71 (1): 016706 (Part 2, Jan. 2005).[PDF]

  • Y. Xu, L.-H. Wang, G. Ambartsoumian and P. Kuchment, "Reconstructions in limited-view thermoacoustic tomography," Medical Physics 31 (4), 724–733 (Apr. 2004).[PDF]

  • Y. Xu and L.-H. Wang, "Time reversal and its application to tomography with diffracting sources," Physical Review Letters 92 (3), 033902 (Jan. 23, 2004).[PDF]

  • X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L.-H. Wang, "Non-invasive laser-induced photoacoustic tomography for structural and functional imaging of the brain in vivo," Nature Biotechnology 21 (7), 803-806 (July 2003). [PDF] Supp. 1. [PDF] Supp. 2. [PDF]

  • M. Xu and L.-H. Wang, "Analytic explanation of spatial resolution related to bandwidth and detector aperture size in thermoacoustic or photoacoustic reconstruction," Physical Review E 67 (5), 056605, 1-15 (May 2003). [PDF]

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Optical Imaging Laboratory at Washington University in St. Louis.